Investigation of lipid peroxidation in sodium lauryl sulfate exposed zebrafish embryos

Abstract

Abstract Aim: Toothpastes are indispensable products to provide personal oral hygiene. Besides their comprehensive benefits, they contain potentially harmful ingredients, including some that they may lead to localized and systemic adverse effects. Some of these effects have been associated with sodium lauryl sulphate (SLS). Being genetically similar to humans zebrafish is used in toxicity potential testing. Zebrafish embryotoxicity test is fast and simple method to study chemical toxicity during embryogenesis. Lipid peroxidation (LPO) is an autoxidation process that is initiated by the attack of free radicals leading to oxidative stress in the pathophysiology of various diseases. The aim of this study was to evaluate lipid peroxidation in SLS exposed zebrafish embryos. Materials and Methods: Adult AB strain zebrafish were used according to Institutional Animal Care and Use Committee protocols. Fish were kept in an aquarium rack system (Zebtec, Tecniplast, Italy). Normally dividing, spherical embryos were exposed to low and high dose of SLS (0,01 mg/10ml and 0,04 mg/10ml) in well plates containing 20 embryos, having 3 replicates. E3 Medium Solution was used for control group. The method of Yagi was used to determine malondialdehyde (MDA) level, an end product of LPO, as thiobarbituric acid reactive substances. LPO was expressed in terms of MDA equivalents as nmol MDA/mg protein. One way ANOVA and Tukey Multiple Comparison Tests were used for statistical analysis. Results: It’s showed that LPO increased significantly in both low (p< 0,05) and high dose SLS (p< 0,05) exposed zebrafish embryos when compared with the control group. Conclusion: LPO may be suggested to be an early indicator of exposure to SLS during embryogenesis and further studies are required to confirm this finding.