Acute toxicity assessment of powdered and liposomal resveratrol that can be used in mineral water

Abstract

Liposomes are now considered the most commonly used nanocarriers for various potentially active hydrophobic and hydrophilic molecules due to their high biocompatibility, biodegradability, and low immunogenicity. Li posomes also proved to enhance drug solubility and controlled distribution, as well as their capacity for surface modifications for targeted, prolonged, and sustained release. Resveratrol (RES) is a polyphenolic compound, has several pharmacologic functions including anti-inflammation and antican cer effects. To investigate the potential therapeutic effects of liposomes and RES, RES-loaded liposomes were produced using the thin-film hydration method. The acute toxicity studies of powdered and liposomal-RES aim to evaluate and investigate their potential toxic effects. The liposomes were characterized by particle size, zeta potential, FTIR, DSC, TEM, LC-MS/MS, and in vitro release kinetics tests. Subsequently, in silico release and distri bution studies were conducted. The in vivo pharmacokinetic parameters of the resveratrol-loaded liposomes were determined, followed by a 28-day acute toxicity study. At the end of the 28-day-period, animals were sacrificed by decapitation, and blood samples, as well as brain, lung, liver, and kidney tissues, will be collected for ELISA/histological analyses. Statistical analyses were performed using the GraphPad software. Initially, the characterization analyses and release tests for the RES-loaded liposomes were successfully completed. In the histopathological study, it was observed that the organs in all treated groups showed no changes at the cellular level in comparison to the control. Histopathological slides also confirmed that no toxicity was ob served in groups treated with different doses of RES-loaded liposomes. Also, the results suggested from our study that the levels or activities of biochem ical parameters in animals have no significant variations occurred in ALT, AST, urea, and creatinine levels at all tested dose in comparison to control. Therefore, the combination of liposomes and resveratrol may offer a novel therapeutic strategy for tissue damage in various disease models.