Cytotoxic activities of Helichrysum arenarium on ECV 304 and Ishikawa cells

Abstract

Introduction: In this study, the cytotoxic activity of Helichrysum arenarium plant on various human cells was examined. Methods: ECV 304 (human endothelial cell line) and Ishikawa cells (human endometrial adenocarcinoma cells) were used in order to find out the cytotoxicity results. Dimethyl sulfoxide (DMSO) extract of Helichrysum arenarium showed the most significant cytotoxicity effect on these cells and also lipid peroxidation (MDA) assay was performed. Also, the essential oils of immortelle extracts were analyzed using gas chromatography-mass spectrometry (GC-MS). Results: Cell viability and MDA levels were decreased by the extract of Helichrysum arenarium both in Ishikawa cells and ECV304 cells at 500 µg/mL. 2-Palmitoylglycerol and palmitic acids were found as the major essential oils in DMSO extracts of Helichrysum arenarium. Conclusion: Helichrysum arenarium may show an antioxidant activity in vitro.