Evaluation of remineralizing potential of hydroxyapatite, phosphopeptide-amorphous calcium phospahate and fluoride dentifrices using SEM/EDX analysis: A randomized controlled in- vitro study

Abstract

Aim: The aim of this study is to evaluate remineralization efficiency of hydroxyapatite (HA) containing dentifrices comparing to phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride (F) containing dentifrices using scanning electron microscope (SEM) and energy dispersive spectrometry (SEM/EDX). Material and methods: 3 x 3 mm2 areas on the buccal surfaces of extracted primary second molars were divided vertically into 3 sections as demineralization, control and reminer-alization surfaces. Control surfaces (1/3 middle) coated without any application. 1/3 left and right surfaces were subjected to demineralization solution. After this application 1/3 right surfaces were coated as demineralization surfaces. Then teeth were randomly separated in 3 groups (n = 10): group HA (Natural toothpaste, Splat (R), Russia); Group CPP-ACP (MI Paste ONE (R), GC, U.S.); group F (Oral-B (R) Kids, Oral-B, Germany). 1/3 right sur-faces were brushed with dentifrices twice a day for one week with pH cycle. Coatings were removed. Surface evaluations were conducted by SEM and EDX. Mann Whitney U and Kruskal Wallis tests were used for statistical analysis. Results: Both atomic (at%) and weight (wt%) percentages of P values were statistically sig-nificant for F group in remineralization surfaces comparing to demineralization surfaces. Ca at%, P at% and P wt% values were statistically significant for CPP-ACP group in remi-neralization surfaces. P at% and P wt% values for F group were higher in remineralization surfaces compared to all. Conclusion: Remineralization was observed in all groups. Most homogeneous surface fea-tures were seen in group CPP-ACP. (c) 2022 Japanese Society of Pediatric Dentistry. Published by Elsevier Ltd. All rights reserved.